Named after Alessandra Zambonelli, from the University of Bologna, mycologist and specialist in hypogeous fungi and mycorrhizae, for her outstanding contribution to the knowledge of Tuber spp.
Ascomata: hypogeous, 0.5–2 cm diam., subglobose, pale brown, smooth, minutely pruinose.
Peridium: 100–200(–300) μm thick, composed of hyaline, agglutinated, interwoven hyphae (intricate texture).
Gleba: firm, solid, whitish at first, becoming pale to dark brown at maturity, marbled with numerous, branching, white and dark veins.
Odour: pleasant.
Tuber zambonelliae grows in Mediterranean Quercus ilex subsp. ballota forests, in limestone mountains of the south of the Iberian Peninsula, 1000–1300 m in altitude. The species occurs in spring and summer.
Tuber zambonelliae is a pale brown truffle that clusters in the rufum clade, and is characterised by its smooth peridium, brown gleba marbled with white and dark veins and spiny-reticulate spores. Tuber zambonelliae resembles Tuber nitidum, but in addition to genetic differences, T. nitidum differs by having a basal cavity, peridium with cellular structure in the outermost layer and smaller spores with separate and shorter spines (Ceruti et al. 2003). Tuber requienii also resembles Tuber zambonelliae but it has a papillose peridium and lacking dark veins (Tulasne & Tulasne 1851).
Asci: inamyloid, 60–70 × 40–50 μm excluding stalk, pyriform to clavate or subglobose, with a long or short stalk arising from a crozier, 10–40 μm long, walls 1–2 μm thick, 1–4(–5)-spored.
Ascospores: 22–45 × 16–26 μm, Q = 1.4–1.9, excluding ornamentation; at first hyaline, yellowish brown at maturity; ellipsoidal to ovoid, ornamented with short spines, 1–2 μm long, often connected by lower ridges, making the ornamentation an irregular and incomplete spiny reticulum.
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